VITRIFICATION WITH CUMULUS CELLS: IMPACT ON FERTILIZATION AND SUBSEQUENT EMBRYO DEVELOPMENT
نویسندگان
چکیده
منابع مشابه
P-29: Effects of Cumulus Cells on Survival, In vitro Maturation,Fertilization and Subsequent Developmental Capacity of Mouse Germinal Vesicle Stage Oocytes during Vitrification
Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...
متن کاملEffects of Vitrification on Immature and in vitro Matured, Denuded and Cumulus Compact Goat Oocytes and Their Subsequent Fertilization
BACKGROUND Vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. The objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. METHODS Oocytes were either cryopreserved as immature cumulus compact (IMCC) (n=98 Exp 1; ...
متن کاملeffects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization
background: vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. the objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. methods: oocytes were either cryopreserved as immature cumulus compact (imcc) (n=98 exp 1; ...
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OBJECTIVE To evaluate the effect of the use of cumulus-aided embryo transfer on pregnancy rates. To study the proximity of expanded cumulus cells to the developing embryo. To document by light microscopy the anchoring of day 3 to day 4 embryos by the expanded cumulus cells. To demonstrate by transmission electron microscopy the cellular activity of the expanded cumulus cells. To evaluate the ex...
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Increased glucocorticoid levels, due to medical therapy or stress-related, may affect reproduction via the hypothalamus-pituitary-axis or directly at the oocyte level. We examined the effects of natural (corticosterone) or synthetic (dexamethasone) glucocorticoids on mouse oocyte maturation and underlying changes in extracellular signal-regulated kinase (ERK) phosphorylation patterns. Fertiliza...
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ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2020
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2020.08.526